Production of rapid qualitative urine hCG results may be used in certain clinical situations requiring pregnancy screening.
Fresh urine, preferably early morning sample or else any urine sample with specific gravity (SG) above 1.005 (measured using the Multstix strips)
Plain white topped universal or paper bedpan.
Samples may be refrigerated for up to 72 hours if the test is not performed immediately.
Blood stained samples are unsuitable for analysis.
A result is produced within 2 5 minutes.
This method uses a cut-off of 25U/L hCG.
Invalid results may be caused by samples which are too dilute or blood stained, the presence of interfering antibodies contained within medications associated with antibody therapies, heterophilic antibodies or non-specific protein binding.
Increased levels of hCG may be present in the urine of non-pregnant women for 2 to 6 weeks after a normal delivery, spontaneous abortion or termination of pregnancy, as the hCG concentration falls to undetectable levels.
If the clinical information is inconsistent with the screen result, if the machine takes a full 5 minutes to give a positive result, or gives a borderline result, a confirmatory serum hCG needs to be sent to the laboratory.
A positive pregnancy screen is not a definitive diagnosis and all positive results should be repeated in 2 3 days or confirmed by serum hCG in the laboratory.
Time Limit for Additional Tests
If the test result is questionable or if clinical signs and symptoms appear inconsistent with the test result, re-test with a fresh early morning sample or confirmation by serum hCG testing in the laboratory.
The test should only be carried out by a trained member of staff.